The Sony SH800Z Sorting Flow Cytometer is a compact, easy to use instrument that performs both cell counting and two-way cell sorting. The integrated laser engine houses 4 lasers, providing excitation lines of 405 nm, 488 nm, 561 nm and 638 nm. These lasers are combined and delivered through a single fiber while 6 freeform PMTs allow for the detection of fluorescence signals from any laser based on filter selection.
| Manufacturer: | Sony |
| Model No.: | SH-800Z |
| Year of manufacture: | 2016 |
| Year acquired: | 2016 |
| Location: | Parsons Room W118 |
The flow cytometer used was purchased by the University of Ò×ʤ²©¹ÙÍø's (Ò×ʤ²©¹ÙÍø's) College of Life Sciences and Agriculture (COLSA) and is supported by Ò×ʤ²©¹ÙÍø's Center for Integrated Biomedical and Bioengineering Research (CIBBR) through a grant from the National Institute of General Medical Sciences (NIGMS) of the National Institutes of Health (NIH) (Grant P20GM113131), and by Ò×ʤ²©¹ÙÍø's University Instrumentation Center (UIC).
Specifications:
- Optics
- Excitation Lasers: 405nm, 488nm, 561nm, 638nm
- Output Power: 30mW (max.) optical fiber output
- Beam Alignment: Collinear optical system
- Detection Parameters: 6 Fluorescence + 2 Light Scatter Channels
- Pulse Measurement: Height, area, width
- Fluidics
- Single, auto-loading tube
- Sample Tube Types: 0.5ml, 1.5ml, 5 ml, 15 ml tubes
- Sort Streams: 2
- Sort Collection Devices: 2-way tube (5ml, 15ml), multiwell plates, PCR tubes, slides
- Temperature Control: Pre-sort -- 5 °C, 37 °C, Post-sort -- 5 °C
- Nozzle Sizes: 70, 100, 130 µm
- Performance
- Maximum event and sorting rate is typically 5,000 events per second.
- Fluorescence wavelengths collected: channel FL1 425-475 nm; FL2 500-550 nm; FL3 570-630; FL4 6560-680; FL5 690-750; FL6 755-815
- Index Sorting: Yes
- Scatter Resolution: 0.5 µm
- Fluorescence Sensitivity: FITC 120 MESF, PE 110 MESF
Principal scientist: Mark Townley
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W123 Parsons Hall
Durham, NH 03824
Phone: (603) 862-3267
Ò×ʤ²©¹ÙÍø Rate:
$59.00/hour assisted
$11.00/hour unassisted
Non Ò×ʤ²©¹ÙÍø academic Rate:
$118.00/hour assisted
$22.00/hour unassisted
Industry Rate:
$150.00/hour assisted
$100.00/hour unassisted
